RIN2 Polyclonal Antibody

Rabbit Anti-RIN2 Polyclonal Antibody

Product Specifications

Background

The RAB5 protein is a small GTPase involved in membrane trafficking in the early endocytic pathway. The protein encoded by RIN2 (Ras And Rab Interactor 2) binds the GTP-bound form of the RAB5 protein preferentially over the GDP-bound form, and functions as a guanine nucleotide exchange factor for RAB5. The encoded protein is found primarily as a tetramer in the cytoplasm and does not bind other members of the RAB family. Mutations in RIN2 cause macrocephaly alopecia cutis laxa and scoliosis (MACS) syndrome, an elastic tissue disorder, as well as the related connective tissue disorder, RIN2 syndrome. Alternative splicing results in multiple transcript variants.

CAS Number

9007-83-4

Product Name Alternative

Ras and Rab interactor 2; Ras association domain family 4; Ras inhibitor JC265; Ras interaction/interference protein 2

Gene ID

54453

Accession Number

Q8WYP3

Reactivity

Human, Mouse

Immunogen

Synthesized peptide derived from part region of human RIN2 protein at amino acid sequence of 70-150

Clonality

Polyclonal

Tag

RIN2

Applications

WB, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

98kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

The antibody detects endogenous levels of RIN2 protein

Observed Molecular Weight

Formulation

Liquid solution

Buffer pH

PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000; ELISA 1:5000-20000