NEK1 Polyclonal Antibody

Rabbit Anti-NEK1 Polyclonal Antibody

Product Specifications

Background

The protein encoded by NEK1 (NIMA Related Kinase 1) is a serine/threonine kinase involved in cell cycle regulation. The encoded protein is found in a centrosomal complex with FEZ1, a neuronal protein that plays a role in axonal development. Defects in NEK1 are a cause of polycystic kidney disease (PKD) . Several transcript variants encoding different isoforms have been found for NEK1.

CAS Number

9007-83-4

Product Name Alternative

Serine/threonine-protein kinase Nek1; Never in mitosis A-related kinase 1; NimA-related protein kinase 1; Renal carcinoma antigen NY-REN-55

Gene ID

4750

Accession Number

Q96PY6

Reactivity

Human, Mouse

Immunogen

Synthesized peptide derived from part region of human NEK1 protein at amino acid sequence of 280-360

Clonality

Polyclonal

Tag

NEK1

Applications

WB, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

138kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

The antibody detects endogenous levels of NEK1 protein

Observed Molecular Weight

138

Formulation

Liquid solution

Buffer pH

PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000; ELISA 1:5000-20000