ASK1 (Phospho Thr838) rabbit pAb

Catalytic activity: ATP + a protein = ADP + a phosphoprotein. Cofactor: Magnesium. enzyme regulation: Contains an N-terminal autoinhibitory domain. Activated by phosphorylation at Thr-838, inhibited by phosphorylation at Ser-966 and Ser-1033. Binds to, and stabilizes MAP3K6 and is activated by MAP3K6 by phosphorylation on Thr-838. function: Component of a protein kinase signal transduction cascade. Phosphorylates and activates MAP2K4 and MAP2K6, which in turn activate the JNK and p38 MAP kinases, respectively. Overexpression induces apoptotic cell death. induction: By TNF-alpha. Inhibited by HIV-1 Nef. similarity: Belongs to the protein kinase superfamily. similarity: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily. similarity: Contains 1 protein kinase domain. subunit: Homodimer when inactive. Binds both upstream activators and downstream substrates in multimolecular complexes. Associates with and inhibited by HIV-1 Nef. Interacts with DAB2IP and PPM1L. tissue specificity: Abundantly expressed in heart and pancreas.

Product Specifications

Background

Catalytic activity:ATP + a protein = ADP + a phosphoprotein., cofactor:Magnesium., enzyme regulation:Contains an N-terminal autoinhibitory domain. Activated by phosphorylation at Thr-838, inhibited by phosphorylation at Ser-966 and Ser-1033. Binds to, and stabilizes MAP3K6 and is activated by MAP3K6 by phosphorylation on Thr-838., function:Component of a protein kinase signal transduction cascade. Phosphorylates and activates MAP2K4 and MAP2K6, which in turn activate the JNK and p38 MAP kinases, respectively. Overexpression induces apoptotic cell death., induction:By TNF-alpha. Inhibited by HIV-1 Nef., similarity:Belongs to the protein kinase superfamily., similarity:Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily., similarity:Contains 1 protein kinase domain., subunit:Homodimer when inactive. Binds both upstream activators and downstream substrates in multimolecular complexes. Associates with and inhibited by HIV-1 Nef. Interacts with DAB2IP and PPM1L., tissue specificity:Abundantly expressed in heart and pancreas.

UniProt

Q99683

Swiss Prot

Q99683

Reactivity

Human; Rat; Mouse

Immunogen

Synthesized peptide derived from human ASK1 (Phospho Thr838)

Clonality

Polyclonal

Source

Rabbit

Applications

WB; ELISA

Concentration

1 mg/ml

Dilution

WB 1:1000-2000 ELISA 1:5000-20000

Molecular Weight

155kD

Storage Conditions

-20°C/1 year

Observed Molecular Weight

155kD

Fragment

IgG

Subcellular Location

Cytoplasm . Endoplasmic reticulum. Interaction with 14-3-3 proteins alters the distribution of MAP3K5/ASK1 and restricts it to the perinuclear endoplasmic reticulum region.

Other Product Names

Mitogen-activated protein kinase kinase kinase 5 (EC 2.7.11.25; Apoptosis signal-regulating kinase 1; ASK-1; MAPK/ERK kinase kinase 5; MEK kinase 5; MEKK 5)