Insulin Polyclonal Antibody
Rabbit Anti-Insulin Polyclonal Antibody
Product Specifications
Background
After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. Alternative splicing results in multiple transcript variants.
CAS Number
9007-83-4
Product Name Alternative
Insulin; Insulin B chain; Insulin A chain
Gene ID
3630
Accession Number
P01308
Reactivity
Human, Mouse, Rat
Immunogen
Synthesized peptide derived from part region of human Insulin protein at amino acid sequence of 30-70
Clonality
Polyclonal
Tag
Insulin
Applications
IHC, IF, ELISA
Purification Method
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Shipping Conditions
Gel pack with blue ice.
Storage Conditions
Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Notes
The antibody detects endogenous levels of Insulin
Formulation
Liquid solution
Buffer pH
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Applications Notes
Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC 1:50-200; ELISA 1:10000-20000; IF 1:50-200
Other Product Names
Insulin; Insulin B chain; Insulin A chain
Tested Applications
WB, IHC, IF, IP, ELISA
Host or Source
Rabbit
Accession Number URL
http://www.uniprot.org/uniprot/P01308
Isotype
Rabbit IgG
Available Sizes
Frequently Asked Questions
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