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GLI-1 Polyclonal Antibody

Rabbit Anti-GLI-1 Polyclonal Antibody

Product Specifications

Background

GLI1 encodes a member of the Kruppel family of zinc finger proteins. The encoded transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop. Multiple transcript variants encoding different isoforms have been found for this gene.

Product Name Alternative

Zinc finger protein GLI1; Glioma-associated oncogene; Oncogene GLI

Gene ID

2735

Accession Number

P08151

Reactivity

Human, Mouse

Immunogen

Synthesized peptide derived from part region of human GLI-1 protein at amino acid sequence of 460-490

Clonality

Polyclonal

Tag

GLI-1

Applications

WB, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

120kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

This antibody detects endogenous levels of GLI-1

Product Datasheet

https://www.abbkine.com/datasheet/ABP58641.pdf

Observed Molecular Weight

120

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000; ELISA 1:10000-20000

Other Product Names

Zinc finger protein GLI1; Glioma-associated oncogene; Oncogene GLI

Tested Applications

WB, IHC, IF, IP, ELISA

Host or Source

Rabbit

Accession Number URL

http://www.uniprot.org/uniprot/P08151

CAS Number

9007-83-4

Isotype

Rabbit IgG

Available Sizes

Curated Selection

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