ARHGF Polyclonal Antibody

Rabbit Anti-ARHGF Polyclonal Antibody

Product Specifications

Background

Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein-coupled receptors. ARHGEF15 (Rho Guanine Nucleotide Exchange Factor 15) encodes a protein that functions as a specific guanine nucleotide exchange factor for RhoA. It also interacts with ephrin A4 in vascular smooth muscle cells. Two alternatively spliced transcripts variants that encode the same protein have been found for ARHGEF15.

CAS Number

9007-83-4

Product Name Alternative

Rho guanine nucleotide exchange factor 15; Ephexin-5; E5; Vsm-RhoGEF

Gene ID

22899

Accession Number

O94989

Reactivity

Human, Mouse

Immunogen

Synthesized peptide derived from part region of human ARHGF protein at amino acid sequence of 560-640

Clonality

Polyclonal

Tag

ARHGF

Applications

WB, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

92kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

The antibody detects endogenous levels of ARHGF protein

Formulation

Liquid solution

Buffer pH

PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000; ELISA 1:5000-20000