Topo IIIα Polyclonal Antibody

Rabbit Anti-Topo IIIα Polyclonal Antibody

Product Specifications

Background

TOP3A (topoisomerase (DNA) III alpha) encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus reducing the number of supercoils and altering the topology of DNA. This enzyme forms a complex with BLM which functions in the regulation of recombination in somatic cells. Alternative splicing results in multiple transcript variants encoding different isoforms.

CAS Number

9007-83-4

Product Name Alternative

TOP3A; TOP3; DNA topoisomerase 3-alpha; DNA topoisomerase III alpha

Gene ID

7156

Accession Number

Q13472

Reactivity

Human, Mouse

Immunogen

Synthesized peptide derived from the Internal region of human Topo IIIα at AA range: 350-430

Clonality

Polyclonal

Tag

DNA topoisomerase 3-alpha

Applications

WB, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

115kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Topo IIIα Polyclonal Antibody detects endogenous levels of Topo IIIα protein.

Observed Molecular Weight

115

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; ELISA 1:10000; Not yet tested in other applications.