Cdc25C (phospho Thr48) Polyclonal Antibody

Rabbit Anti-Cdc25C (phospho Thr48) Polyclonal Antibody

Product Specifications

Background

CDC25C (cell division cycle 25C) encodes a conserved protein that plays a key role in the regulation of cell division. The encoded protein directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It also suppresses p53-induced growth arrest. Multiple alternatively spliced transcript variants of CDC25C have been described.

CAS Number

9007-83-4

Product Name Alternative

CDC25C; M-phase inducer phosphatase 3; Dual specificity phosphatase Cdc25C

Gene ID

995

Accession Number

P30307

Reactivity

Human, Rat, Mouse

Immunogen

Synthesized peptide derived from human Cdc25C around the phosphorylation site of T48

Clonality

Polyclonal

Tag

M-phase inducer phosphatase 3

Applications

WB, IHC, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

80kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Phospho-Cdc25C (T48) Polyclonal Antibody detects endogenous levels of Cdc25C protein only when phosphorylated at T48.

Observed Molecular Weight

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:5000; IF 1:50-200