Cdc6 (phospho Ser54) Polyclonal Antibody

Rabbit Anti-Cdc6 (phospho Ser54) Polyclonal Antibody

Product Specifications

Background

The protein encoded by CDC6 (cell division cycle 6) is highly similar to Saccharomyces cerevisiae Cdc6, a protein essential for the initiation of DNA replication. This protein functions as a regulator at the early steps of DNA replication. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Transcription of this protein was reported to be regulated in response to mitogenic signals through transcriptional control mechanism involving E2F proteins.

CAS Number

9007-83-4

Product Name Alternative

CDC6; CDC18L; Cell division control protein 6 homolog; CDC6-related protein; Cdc18-related protein; HsCdc18; p62 (cdc6) ; HsCDC6

Gene ID

990

Accession Number

Q99741

Reactivity

Human, Mouse, Monkey

Immunogen

Synthesized peptide derived from human Cdc6 around the phosphorylation site of S54

Clonality

Polyclonal

Tag

Cell division control protein 6 homolog

Applications

WB, IHC, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

62kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Phospho-Cdc6 (S54) Polyclonal Antibody detects endogenous levels of Cdc6 protein only when phosphorylated at S54.

Observed Molecular Weight

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:5000; IF 1:50-200