ACAP1 (phospho Ser554) Polyclonal Antibody

ACAP1, also designated Centaurin-β 1 (CENTB1 or Cnt-β1), is a member of the ADP ribosylation factor family of ARF6 GTPase-activating proteins (GAP) . GAPs are important regulators of Arf function by controlling the return of ARF to its inactive state. ACAP1 is related to AGAP1 and ASAP1, and all three proteins are similarly expressed in fibroblast cells such as NIH/3T3. Internalization and recycling of integrin receptors is important in cell adhesion and migration modulation, and once inside a cell, proteins and membranes are transported to the endosome where they are sorted for recycling or degradation. ACAP1 promotes cargo sorting by associating directly to recycling cargo proteins. Preventing this interaction inhibits protein recycling. ACAP1 binds transferrin receptors, promoting their transport to the plasma membrane from the endosome. Akt induced phosphorylation of ACAP1 at Ser 554 regulates ACAP1 interaction to integrin in endosomes, and downregulation of Akt or ACAP1 may inhibit cell migration on Fibronectin.

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Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000; WB 1:500-2000; IHC 1:100-1:300; ELISA 1:10000-40000; IF 1:50-200