MDC1 (phospho Ser513) Polyclonal Antibody

Rabbit Anti-MDC1 (phospho Ser513) Polyclonal Antibody

Product Specifications

Background

The protein encoded by MDC1 (mediator of DNA damage checkpoint 1) contains an N-terminal forkhead domain, two BRCA1 C-terminal (BRCT) motifs and a central domain with 13 repetitions of an approximately 41-amino acid sequence. The encoded protein is required to activate the intra-S phase and G2/M phase cell cycle checkpoints in response to DNA damage. This nuclear protein interacts with phosphorylated histone H2AX near sites of DNA double-strand breaks through its BRCT motifs, and facilitates recruitment of the ATM kinase and meiotic recombination 11 protein complex to DNA damage foci.

CAS Number

9007-83-4

Product Name Alternative

MDC1; KIAA0170; NFBD1; Mediator of DNA damage checkpoint protein 1; Nuclear factor with BRCT domains 1

Gene ID

9656

Accession Number

Q14676

Reactivity

Human, Rat, Mouse

Immunogen

Synthesized peptide derived from human MDC1 around the phosphorylation site of S513

Clonality

Polyclonal

Tag

Mediator of DNA damage checkpoint protein 1

Applications

IHC, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Phospho-MDC1 (S513) Polyclonal Antibody detects endogenous levels of MDC1 protein only when phosphorylated at S513.

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC 1:100-1:300; ELISA 1:5000; IF 1:50-200