CPI-17 (phospho Thr38) Polyclonal Antibody

Rabbit Anti-CPI-17 (phospho Thr38) Polyclonal Antibody

Product Specifications

Background

The protein encoded by PPP1R14A (protein phosphatase 1 regulatory inhibitor subunit 14A) belongs to the protein phosphatase 1 (PP1) inhibitor family. This protein is an inhibitor of smooth muscle myosin phosphatase, and has higher inhibitory activity when phosphorylated. Inhibition of myosin phosphatase leads to increased myosin phosphorylation and enhanced smooth muscle contraction. Alternatively spliced transcript variants encoding different isoforms have been noted for PPP1R14A.

CAS Number

9007-83-4

Product Name Alternative

PPP1R14A; CPI17; PPP1INL; Protein phosphatase 1 regulatory subunit 14A; 17 kDa PKC-potentiated inhibitory protein of PP1; Protein kinase C-potentiated inhibitor protein of 17 kDa; CPI-17

Gene ID

94274

Accession Number

Q96A00

Reactivity

Human, Mouse, Rat

Immunogen

Synthesized peptide derived from human CPI-17 around the phosphorylation site of T38

Clonality

Polyclonal

Tag

Protein phosphatase 1 regulatory subunit 14A

Applications

WB, IHC, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

22kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Phospho-CPI-17 (T38) Polyclonal Antibody detects endogenous levels of CPI-17 protein only when phosphorylated at T38.

Observed Molecular Weight

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:20000; IF 1:50-200

Other Product Names

PPP1R14A; CPI17; PPP1INL; Protein phosphatase 1 regulatory subunit 14A; 17 kDa PKC-potentiated inhibitory protein of PP1; Protein kinase C-potentiated inhibitor protein of 17 kDa; CPI-17