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CD28 (phospho Tyr218) Polyclonal Antibody

Rabbit Anti-CD28 (phospho Tyr218) Polyclonal Antibody

Product Specifications

Background

The protein encoded by CD28 (CD28 molecule) is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for CD28.

Product Name Alternative

CD28; T-cell-specific surface glycoprotein CD28; TP44; CD antigen CD28

Gene ID

940

Accession Number

P10747

Reactivity

Human

Immunogen

Synthesized peptide derived from human CD28 around the phosphorylation site of Y218

Clonality

Polyclonal

Tag

T-cell-specific surface glycoprotein CD28

Applications

WB, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

45kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Phospho-CD28 (Y218) Polyclonal Antibody detects endogenous levels of CD28 protein only when phosphorylated at Y218.

Product Datasheet

https://www.abbkine.com/datasheet/ABP57033.pdf

Observed Molecular Weight

45

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; ELISA 1:40000; Not yet tested in other applications.

Other Product Names

CD28; T-cell-specific surface glycoprotein CD28; TP44; CD antigen CD28

Tested Applications

WB, IHC, IF, IP, ELISA

Host or Source

Rabbit

Accession Number URL

http://www.uniprot.org/uniprot/P10747

CAS Number

9007-83-4

Isotype

Rabbit IgG

Available Sizes

Curated Selection

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