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MAPKAPK-2 (phospho Thr222) Polyclonal Antibody

Rabbit Anti-MAPKAPK-2 (phospho Thr222) Polyclonal Antibody

Product Specifications

Background

MAPKAPK2 (mitogen-activated protein kinase-activated protein kinase 2) encodes a member of the Ser/Thr protein kinase family. This kinase is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for MAPKAPK2.

Product Name Alternative

MAPKAPK2; MAP kinase-activated protein kinase 2; MAPK-activated protein kinase 2; MAPKAP kinase 2; MAPKAP-K2; MAPKAPK-2; MK-2; MK2

Gene ID

9261

Accession Number

P49137

Reactivity

Human, Mouse, Rat, Monkey

Immunogen

Synthesized peptide derived from human MAPKAPK-2 around the phosphorylation site of T222

Clonality

Polyclonal

Tag

MAP kinase-activated protein kinase 2

Applications

WB, IHC, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

45kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Phospho-MAPKAPK-2 (T222) Polyclonal Antibody detects endogenous levels of MAPKAPK-2 protein only when phosphorylated at T222.

Product Datasheet

https://www.abbkine.com/datasheet/ABP57005.pdf

Observed Molecular Weight

45

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:20000; IF 1:50-200

Other Product Names

MAPKAPK2; MAP kinase-activated protein kinase 2; MAPK-activated protein kinase 2; MAPKAP kinase 2; MAPKAP-K2; MAPKAPK-2; MK-2; MK2

Tested Applications

WB, IHC, IF, IP, ELISA

Host or Source

Rabbit

Accession Number URL

http://www.uniprot.org/uniprot/P49137

CAS Number

9007-83-4

Isotype

Rabbit IgG

Available Sizes

Curated Selection

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