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Cyclin B1 (phospho Ser147) Polyclonal Antibody

Rabbit Anti-Cyclin B1 (phospho Ser147) Polyclonal Antibody

Product Specifications

Background

The protein encoded by CCNB1 (cyclin B1) is a regulatory protein involved in mitosis. The gene product complexes with p34 (cdc2) to form the maturation-promoting factor (MPF) . Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.

CAS Number

9007-83-4

Product Name Alternative

CCNB1; CCNB; G2/mitotic-specific cyclin-B1

Gene ID

891

Accession Number

P14635

Reactivity

Human, Mouse, Rat

Immunogen

Synthesized peptide derived from human Cyclin B1 around the phosphorylation site of S147

Clonality

Polyclonal

Tag

Cyclin-B1

Applications

WB, IHC, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

60kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Phospho-Cyclin B1 (S147) Polyclonal Antibody detects endogenous levels of Cyclin B1 protein only when phosphorylated at S147.

Product Datasheet

https://www.abbkine.com/datasheet/ABP56907.pdf

Observed Molecular Weight

60

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:10000; IF 1:50-200

Other Product Names

CCNB1; CCNB; G2/mitotic-specific cyclin-B1

Tested Applications

WB, IHC, IF, IP, ELISA

Host or Source

Rabbit

Accession Number URL

http://www.uniprot.org/uniprot/P14635

Isotype

Rabbit IgG

Available Sizes

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