HDAC3 (phospho Ser424) Polyclonal Antibody

Rabbit Anti-HDAC3 (phospho Ser424) Polyclonal Antibody

Product Specifications

Background

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by HDAC3 (histone deacetylase 3) belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. HDAC3 is regarded as a potential tumor suppressor gene.

CAS Number

9007-83-4

Product Name Alternative

HDAC3; Histone deacetylase 3; HD3; RPD3-2; SMAP45

Gene ID

8841

Accession Number

O15379

Reactivity

Human, Mouse, Rat

Immunogen

Synthesized peptide derived from human HDAC3 around the phosphorylation site of S424

Clonality

Polyclonal

Tag

Histone deacetylase 3

Applications

WB, IHC, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

48kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Phospho-HDAC3 (S424) Polyclonal Antibody detects endogenous levels of HDAC3 protein only when phosphorylated at S424.

Observed Molecular Weight

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:10000; Not yet tested in other applications.