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β-1, 3-Gal-T2 Polyclonal Antibody

Rabbit Anti-β-1,3-Gal-T2 Polyclonal Antibody

Product Specifications

Background

B3GALT2 (beta-1,3-galactosyltransferase 2) is a member of the beta-1,3-galactosyltransferase (beta3GalT) gene family. This family encodes type II membrane-bound glycoproteins with diverse enzymatic functions using different donor substrates (UDP-galactose and UDP-N-acetylglucosamine) and different acceptor sugars (N-acetylglucosamine, galactose, N-acetylgalactosamine) . The beta3GalT genes are distantly related to the Drosophila Brainiac gene and have the protein coding sequence contained in a single exon. The beta3GalT proteins also contain conserved sequences not found in the beta4GalT or alpha3GalT proteins. The carbohydrate chains synthesized by these enzymes are designated as type 1, whereas beta4GalT enzymes synthesize type 2 carbohydrate chains. The ratio of type 1: type 2 chains changes during embryogenesis. By sequence similarity, the beta3GalT genes fall into at least two groups: beta3GalT4 and 4 other beta3GalT genes (beta3GalT1-3, beta3GalT5) . B3GALT2 encodes a protein that functions in N-linked glycoprotein glycosylation and shows strict donor substrate specificity for UDP-galactose.

CAS Number

9007-83-4

Product Name Alternative

B3GALT2; Beta-1; 3-galactosyltransferase 2; Beta-1,3-GalTase 2; Beta3Gal-T2; Beta3GalT2; UDP-galactose:2-acetamido-2-deoxy-D-glucose 3beta-galactosyltransferase 2

Gene ID

8707

Accession Number

O43825

Reactivity

Human, Mouse

Immunogen

Synthesized peptide derived from the C-terminal region of human β-1,3-Gal-T2 at AA range: 350-430

Clonality

Polyclonal

Tag

Beta-1,3-galactosyltransferase 2

Applications

IHC, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

β-1,3-Gal-T2 Polyclonal Antibody detects endogenous levels of β-1,3-Gal-T2 protein.

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:40000; Not yet tested in other applications.

Other Product Names

B3GALT2; Beta-1; 3-galactosyltransferase 2; Beta-1,3-GalTase 2; Beta3Gal-T2; Beta3GalT2; UDP-galactose:2-acetamido-2-deoxy-D-glucose 3beta-galactosyltransferase 2

Tested Applications

WB, IHC, IF, IP, ELISA

Host or Source

Rabbit

Accession Number URL

http://www.uniprot.org/uniprot/O43825

Isotype

Rabbit IgG

Available Sizes

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