Mnk1 (phospho Thr385) Polyclonal Antibody

Rabbit Anti-Mnk1 (phospho Thr385) Polyclonal Antibody

Product Specifications

Background

MKNK1 (MAP kinase interacting serine/threonine kinase 1) encodes a Ser/Thr protein kinase that interacts with, and is activated by ERK1 and p38 mitogen-activated protein kinases, and thus may play a role in the response to environmental stress and cytokines. This kinase may also regulate transcription by phosphorylating eIF4E via interaction with the C-terminal region of eIF4G. Alternatively spliced transcript variants have been noted for MKNK1.

CAS Number

9007-83-4

Product Name Alternative

MKNK1; MNK1; MAP kinase-interacting serine/threonine-protein kinase 1; MAP kinase signal-integrating kinase 1; MAPK signal-integrating kinase 1; Mnk1

Gene ID

8569

Accession Number

Q9BUB5

Reactivity

Human, Mouse, Rat

Immunogen

Synthesized peptide derived from human Mnk1 around the phosphorylation site of T385

Clonality

Polyclonal

Tag

MAP kinase-interacting serine threonine-protein kinase 1

Applications

WB, IHC, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

42kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Phospho-Mnk1 (T385) Polyclonal Antibody detects endogenous levels of Mnk1 protein only when phosphorylated at T385.

Observed Molecular Weight

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:20000; IF 1:50-200