MYPT3 Polyclonal Antibody

Rabbit Anti-MYPT3 Polyclonal Antibody

Product Specifications

Background

Myosin light chain kinase and phosphatase (MLCP) complexes control the phosphorylation states of regulatory myosin light chains, which is crucial for muscle and intracellular movement. MLCPs typically contain a catalytic protein phosphatase 1 (PP1c) subunit, a myosin phosphatase targeting (MYPT) subunit, and another smaller subunit. The protein encoded by MYPT3 (protein phosphatase 1 regulatory subunit 16A, PPP1R16A) represents an MYPT subunit, which is responsible for directing PP1c to its intended targets. However, while other MYPTs result in PP1c activation after becoming phosphorylated, the encoded protein is phosphorylated by protein kinase A and then inhibits the catalytic activity of PP1c.

CAS Number

9007-83-4

Product Name Alternative

PPP1R16A; MYPT3; Protein phosphatase 1 regulatory subunit 16A; Myosin phosphatase-targeting subunit 3

Gene ID

84988

Accession Number

Q96I34

Reactivity

Human, Mouse

Immunogen

Synthesized peptide derived from the Internal region of human MYPT3 at AA range: 340-420

Clonality

Polyclonal

Tag

Protein phosphatase 1 regulatory subunit 16A

Applications

WB, IF, ELISA, IHC

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

60kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

MYPT3 Polyclonal Antibody detects endogenous levels of MYPT3 protein.

Observed Molecular Weight

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000; IF/ICC1:50-200; ELISA 1:2000-20000; IHC 1:50-200