CaSR (phospho Thr888) Polyclonal Antibody

Rabbit Anti-CaSR (phospho Thr888) Polyclonal Antibody

Product Specifications

Background

The protein encoded by CASR (calcium sensing receptor) is a G protein-coupled receptor that is expressed in the parathyroid hormone (PTH) -producing chief cells of the parathyroid gland, and the cells lining the kidney tubule. It senses small changes in circulating calcium concentration and couples this information to intracellular signaling pathways that modify PTH secretion or renal cation handling, thus this protein plays an essential role in maintaining mineral ion homeostasis. Mutations in CASR cause familial hypocalciuric hypercalcemia, familial, isolated hypoparathyroidism, and neonatal severe primary hyperparathyroidism.

CAS Number

9007-83-4

Product Name Alternative

CASR; GPRC2A; PCAR1; Extracellular calcium-sensing receptor; CaSR; Parathyroid cell calcium-sensing receptor; PCaR1

Gene ID

846

Accession Number

P41180

Reactivity

Human, Mouse, Rat

Immunogen

Synthesized peptide derived from human CaSR around the phosphorylation site of T888

Clonality

Polyclonal

Tag

Extracellular calcium-sensing receptor

Applications

WB, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

140kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Phospho-CaSR (T888) Polyclonal Antibody detects endogenous levels of CaSR protein only when phosphorylated at T888.

Observed Molecular Weight

140

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IF 1:200-1:1000; ELISA 1:40000; Not yet tested in other applications.