Wee1 (phospho Ser642) Polyclonal Antibody

Rabbit Anti-Wee1 (phospho Ser642) Polyclonal Antibody

Product Specifications

Background

WEE1 (WEE1 G2 checkpoint kinase) encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase.

CAS Number

9007-83-4

Product Name Alternative

WEE1; Wee1-like protein kinase; WEE1hu; Wee1A kinase

Gene ID

7465

Accession Number

P30291

Reactivity

Human, Mouse, Rat

Immunogen

Synthesized peptide derived from human Wee1 around the phosphorylation site of S642

Clonality

Polyclonal

Tag

Wee1-like protein kinase

Applications

WB, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

100kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Phospho-Wee1 (S642) Polyclonal Antibody detects endogenous levels of Wee1 protein only when phosphorylated at S642.

Observed Molecular Weight

100

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; ELISA 1:5000; Not yet tested in other applications.

Other Product Names

WEE1; Wee1-like protein kinase; WEE1hu; Wee1A kinase

Tested Applications

WB, IHC, IF, IP, ELISA

Host or Source

Rabbit

Accession Number URL

http://www.uniprot.org/uniprot/P30291

Isotype

Rabbit IgG

Available Sizes

Curated Selection

Explore Other Products

Discover premium biology products from our extensive collection of 20M+ items

ARG29014 1 Million

GNS Knockout A2780 Polyclonal Cells

Ask

View Details

MR226780 10 µg

Ccdc160 (NM_001034059) Mouse Tagged ORF Clone

Ask

View Details

124137-APC 100 µL

C4BPB (C4b-binding Protein beta Chain) (APC)

Ask

View Details

RC225914L3 10 µg

ARHGEF3 (NM_001128616) Human Tagged Lenti ORF Clone

Ask

View Details