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Ezrin (phospho Tyr478) Polyclonal Antibody

Rabbit Anti-Ezrin (phospho Tyr478) Polyclonal Antibody

Product Specifications

Background

The cytoplasmic peripheral membrane protein encoded by EZR (ezrin) functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for EZR. Alternatively spliced variants have also been described for EZR.

Product Name Alternative

EZR; VIL2; Ezrin; Cytovillin; Villin-2; p81

Gene ID

7430

Accession Number

P15311

Reactivity

Human, Mouse, Rat

Immunogen

Synthesized peptide derived from human Ezrin around the phosphorylation site of Y478

Clonality

Polyclonal

Tag

Ezrin

Applications

WB, IHC, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

70kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Phospho-Ezrin (Y478) Polyclonal Antibody detects endogenous levels of Ezrin protein only when phosphorylated at Y478.

Product Datasheet

https://www.abbkine.com/datasheet/ABP56504.pdf

Observed Molecular Weight

70

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:5000; IF 1:50-200

Other Product Names

EZR; VIL2; Ezrin; Cytovillin; Villin-2; p81

Tested Applications

WB, IHC, IF, IP, ELISA

Host or Source

Rabbit

Accession Number URL

http://www.uniprot.org/uniprot/P15311

CAS Number

9007-83-4

Isotype

Rabbit IgG

Available Sizes

Curated Selection

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