Tuberin (phospho Thr1462) Polyclonal Antibody

Rabbit Anti-Tuberin (phospho Thr1462) Polyclonal Antibody

Product Specifications

Background

Mutations in TSC2 (tuberous sclerosis 2) lead to tuberous sclerosis complex. Its gene product is believed to be a tumor suppressor and is able to stimulate specific GTPases. The protein associates with hamartin in a cytosolic complex, possibly acting as a chaperone for hamartin. Alternative splicing results in multiple transcript variants encoding different isoforms.

CAS Number

9007-83-4

Product Name Alternative

TSC2; TSC4; Tuberin; Tuberous sclerosis 2 protein

Gene ID

7249

Accession Number

P49815

Reactivity

Human, Mouse, Rat

Immunogen

Synthesized peptide derived from human Tuberin around the phosphorylation site of T1462

Clonality

Polyclonal

Tag

Tuberin

Applications

WB, IHC, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

200kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Phospho-Tuberin (T1462) Polyclonal Antibody detects endogenous levels of Tuberin protein only when phosphorylated at T1462.

Observed Molecular Weight

200

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:5000; Not yet tested in other applications.