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Troponin I-C (phospho Thr142) Polyclonal Antibody

Rabbit Anti-Troponin I-C (phospho Thr142) Polyclonal Antibody

Product Specifications

Background

Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: tnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. NNI3 (troponin I3, cardiac type) encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in TNNI3 cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM) .

CAS Number

9007-83-4

Product Name Alternative

TNNI3; TNNC1; Troponin I; cardiac muscle; Cardiac troponin I

Gene ID

7137

Accession Number

P19429

Reactivity

Human, Mouse, Rat

Immunogen

Synthesized peptide derived from human Troponin I-C around the phosphorylation site of T142

Clonality

Polyclonal

Tag

Troponin I cardiac muscle

Applications

WB, IHC, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

28kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Phospho-Troponin I-C (T142) Polyclonal Antibody detects endogenous levels of Troponin I-C protein only when phosphorylated at T142.

Observed Molecular Weight

28

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; ELISA 1:20000; IF 1:50-200

Other Product Names

TNNI3; TNNC1; Troponin I; cardiac muscle; Cardiac troponin I

Tested Applications

WB, IHC, IF, IP, ELISA

Host or Source

Rabbit

Accession Number URL

http://www.uniprot.org/uniprot/P19429

Isotype

Rabbit IgG

Available Sizes

Frequently Asked Questions

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