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Claudin-5 (phospho Tyr217) Polyclonal Antibody

Rabbit Anti-Claudin-5 (phospho Tyr217) Polyclonal Antibody

Product Specifications

Background

CLDN5 (claudin 5) encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets. Mutations in CLDN5 have been found in patients with velocardiofacial syndrome. Alternatively spliced transcript variants encoding the same protein have been found for CLDN5.

Product Name Alternative

CLDN5; AWAL; TMVCF; Claudin-5; Transmembrane protein deleted in VCFS; TMDVCF

Gene ID

7122

Accession Number

O00501

Reactivity

Human, Mouse, Rat

Immunogen

Synthesized peptide derived from human Claudin-5 around the phosphorylation site of Y217

Clonality

Polyclonal

Tag

Claudin-5

Applications

IHC, IF, WB, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

23kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Phospho-Claudin-5 (Y217) Polyclonal Antibody detects endogenous levels of Claudin-5 protein only when phosphorylated at Y217.

Product Datasheet

https://www.abbkine.com/datasheet/ABP56401.pdf

Observed Molecular Weight

23

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000; IHC 1:100-1:300; ELISA 1:5000; IF 1:50-200

Other Product Names

CLDN5; AWAL; TMVCF; Claudin-5; Transmembrane protein deleted in VCFS; TMDVCF

Tested Applications

WB, IHC, IF, IP, ELISA

Host or Source

Rabbit

Accession Number URL

http://www.uniprot.org/uniprot/O00501

CAS Number

9007-83-4

Isotype

Rabbit IgG

Available Sizes

Curated Selection

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