C-Src (phospho Tyr216) Polyclonal Antibody

Rabbit Anti-c-Src (phospho Tyr216) Polyclonal Antibody

Product Specifications

Background

SRC (SRC proto-oncogene, non-receptor tyrosine kinase) is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by SRC is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in SRC could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for SRC.

CAS Number

9007-83-4

Product Name Alternative

SRC; SRC1; Proto-oncogene tyrosine-protein kinase Src; Proto-oncogene c-Src; pp60c-src; p60-Src

Gene ID

6714

Accession Number

P12931

Reactivity

Human, Mouse, Rat

Immunogen

Synthesized peptide derived from human c-Src around the phosphorylation site of Y216

Clonality

Polyclonal

Tag

Proto-oncogene tyrosine-protein kinase Src

Applications

WB, IHC, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

50kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Phospho-c-Src (Y216) Polyclonal Antibody detects endogenous levels of c-Src protein only when phosphorylated at Y216.

Observed Molecular Weight

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:10000; IF 1:50-200