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SP-100 Polyclonal Antibody

Rabbit Anti-SP-100 Polyclonal Antibody

Product Specifications

Background

SP100 (SP100 nuclear antigen) encodes a subnuclear organelle and major component of the PML (promyelocytic leukemia) -SP100 nuclear bodies. PML and SP100 are covalently modified by the SUMO-1 modifier, which is considered crucial to nuclear body interactions. The encoded protein binds heterochromatin proteins and is thought to play a role in tumorigenesis, immunity, and gene regulation. Alternatively spliced variants have been identified for SP100; one of which encodes a high-mobility group protein.

CAS Number

9007-83-4

Product Name Alternative

SP100; Nuclear autoantigen Sp-100; Lysp100b; Nuclear dot-associated Sp100 protein; Speckled 100 kDa

Gene ID

6672

Accession Number

P23497

Reactivity

Human, Mouse

Immunogen

Synthesized peptide derived from the Internal region of human SP-100 at AA range: 250-330

Clonality

Polyclonal

Tag

Nuclear autoantigen Sp-100

Applications

WB, IHC, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

100kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

SP-100 Polyclonal Antibody detects endogenous levels of SP-100 protein.

Observed Molecular Weight

100

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:40000; IF 1:50-200

Other Product Names

SP100; Nuclear autoantigen Sp-100; Lysp100b; Nuclear dot-associated Sp100 protein; Speckled 100 kDa

Tested Applications

WB, IHC, IF, IP, ELISA

Host or Source

Rabbit

Accession Number URL

http://www.uniprot.org/uniprot/P23497

Isotype

Rabbit IgG

Available Sizes

Frequently Asked Questions

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