NBK (phospho Ser35) Polyclonal Antibody

Rabbit Anti-NBK (phospho Ser35) Polyclonal Antibody

Product Specifications

Background

The protein encoded by BIK (BCL2 interacting killer) shares a critical BH3 domain with other death-promoting proteins, such as BID, BAK, BAD and BAX, that is required for its pro-apoptotic activity, and for interaction with anti-apoptotic members of the BCL2 family, and viral survival-promoting proteins. Since the activity of this protein is suppressed in the presence of survival-promoting proteins, it is suggested as a likely target for anti-apoptotic proteins.

CAS Number

9007-83-4

Product Name Alternative

BIK; NBK; Bcl-2-interacting killer; Apoptosis inducer NBK; BIP1; BP4

Gene ID

638

Accession Number

Q13323

Reactivity

Human, Rat, Mouse

Immunogen

Synthesized peptide derived from human NBK around the phosphorylation site of S35

Clonality

Polyclonal

Tag

Bcl-2-interacting killer

Applications

IHC, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Phospho-NBK (S35) Polyclonal Antibody detects endogenous levels of NBK protein only when phosphorylated at S35.

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC 1:100-1:300; ELISA 1:5000; IF 1:50-200