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ENaC β (phospho Thr615) Polyclonal Antibody

Rabbit Anti-ENaC β (phospho Thr615) Polyclonal Antibody

Product Specifications

Background

Nonvoltage-gated, amiloride-sensitive, sodium channels control fluid and electrolyte transport across epithelia in many organs. These channels are heteromeric complexes consisting of 3 subunits: alpha, beta, and gamma. SCNN1B (sodium channel epithelial 1 beta subunit) encodes the beta subunit, and mutations in SCNN1B have been associated with pseudohypoaldosteronism type 1 (PHA1), and Liddle syndrome.

Product Name Alternative

SCNN1B; Amiloride-sensitive sodium channel subunit beta; Beta-NaCH; Epithelial Na (+) channel subunit beta; Beta-ENaC; ENaCB; Nonvoltage-gated sodium channel 1 subunit beta; SCNEB

Gene ID

6338

Accession Number

P51168

Reactivity

Human, Mouse, Rat

Immunogen

Synthesized peptide derived from human ENaC β around the phosphorylation site of T615

Clonality

Polyclonal

Tag

Amiloride-sensitive sodium channel subunit beta

Applications

WB, IHC, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

68kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Phospho-ENaC β (T615) Polyclonal Antibody detects endogenous levels of ENaC β protein only when phosphorylated at T615.

Product Datasheet

https://www.abbkine.com/datasheet/ABP56126.pdf

Observed Molecular Weight

68

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:10000; Not yet tested in other applications.

Other Product Names

SCNN1B; Amiloride-sensitive sodium channel subunit beta; Beta-NaCH; Epithelial Na (+) channel subunit beta; Beta-ENaC; ENaCB; Nonvoltage-gated sodium channel 1 subunit beta; SCNEB

Tested Applications

WB, IHC, IF, IP, ELISA

Host or Source

Rabbit

Accession Number URL

http://www.uniprot.org/uniprot/P51168

CAS Number

9007-83-4

Isotype

Rabbit IgG

Available Sizes

Curated Selection

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