Rsk-1/2/3/4 (phospho Ser221/227/S218/232) Polyclonal Antibody

Rabbit Anti-Rsk-1/2/3/4 (phospho Ser221/227/S218/232) Polyclonal Antibody

Product Specifications

Background

RPS6KA1 (ribosomal protein S6 kinase A1) encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

CAS Number

9007-83-4

Product Name Alternative

RPS6KA1; MAPKAPK1A; RSK1; Ribosomal protein S6 kinase alpha-1; S6K-alpha-1; 90 kDa ribosomal protein S6 kinase 1; p90-RSK 1; p90RSK1; p90S6K; MAP kinase-activated protein kinase 1a; MAPK-activated protein kinase 1a; MAPKAP kinase 1a; MAPKAP

Gene ID

6195/6197/6196/27330

Accession Number

Q15418/P51812/Q15349/Q9UK32

Reactivity

Human, Mouse

Immunogen

Synthesized peptide derived from human Rsk-1/2/3/4 around the phosphorylation site of S221/227/S218/232

Clonality

Polyclonal

Tag

Ribosomal protein S6 kinase alpha-1

Applications

WB, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

85kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Phospho-Rsk-1/2/3/4 (S221/227/S218/232) Polyclonal Antibody detects endogenous levels of Rsk-1/2/3/4 protein only when phosphorylated at S221/227/S218/232.

Observed Molecular Weight

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; ELISA 1:40000; Not yet tested in other applications.