Rb (phospho Thr826) Polyclonal Antibody

Rabbit Anti-Rb (phospho Thr826) Polyclonal Antibody

Product Specifications

Background

The protein encoded by RB1 (RB transcriptional corepressor 1) is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in RB1 are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma.

CAS Number

9007-83-4

Product Name Alternative

RB1; Retinoblastoma-associated protein; p105-Rb; pRb; Rb; pp110

Gene ID

5925

Accession Number

P06400

Reactivity

Human, Mouse, Rat

Immunogen

Synthesized peptide derived from human Rb around the phosphorylation site of T826

Clonality

Polyclonal

Tag

Retinoblastoma-associated protein

Applications

WB, IHC, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

110kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Phospho-Rb (T826) Polyclonal Antibody detects endogenous levels of Rb protein only when phosphorylated at T826.

Observed Molecular Weight

110

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:10000; Not yet tested in other applications.