Brk (phospho Tyr447) Polyclonal Antibody

Rabbit Anti-Brk (phospho Tyr447) Polyclonal Antibody

Product Specifications

Background

The protein encoded by PTK6 (protein tyrosine kinase 6) is a cytoplasmic nonreceptor protein kinase which may function as an intracellular signal transducer in epithelial tissues. Overexpression of PTK6 in mammary epithelial cells leads to sensitization of the cells to epidermal growth factor and results in a partially transformed phenotype. Expression of PTK6 has been detected at low levels in some breast tumors but not in normal breast tissue. The encoded protein has been shown to undergo autophosphorylation. Alternative splicing results in multiple transcript variants.

CAS Number

9007-83-4

Product Name Alternative

PTK6; BRK; Protein-tyrosine kinase 6; Breast tumor kinase; Tyrosine-protein kinase BRK

Gene ID

5753

Accession Number

Q13882

Reactivity

Human, Rat, Mouse

Immunogen

Synthesized peptide derived from human Brk around the phosphorylation site of Y447

Clonality

Polyclonal

Tag

Protein-tyrosine kinase 6

Applications

WB, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

50kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Phospho-Brk (Y447) Polyclonal Antibody detects endogenous levels of Brk protein only when phosphorylated at Y447.

Observed Molecular Weight

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IF 1:200-1:1000; ELISA 1:5000; Not yet tested in other applications.