PKR (phospho Thr451) Polyclonal Antibody
Rabbit Anti-PKR (phospho Thr451) Polyclonal Antibody
Product Specifications
Background
The protein encoded by EIF2AK2 (eukaryotic translation initiation factor 2 alpha kinase 2) is a serine/threonine protein kinase that is activated by autophosphorylation after binding to dsRNA. The activated form of the encoded protein can phosphorylate translation initiation factor EIF2S1, which in turn inhibits protein synthesis. This protein is also activated by manganese ions and heparin. Three transcript variants encoding two different isoforms have been found for EIF2AK2.
CAS Number
9007-83-4
Product Name Alternative
EIF2AK2; PKR; PRKR; Interferon-induced; double-stranded RNA-activated protein kinase; Eukaryotic translation initiation factor 2-alpha kinase 2; eIF-2A protein kinase 2; Interferon-inducible RNA-dependent protein kinase; P1/eIF-2A protein k
Gene ID
5610
Accession Number
P19525
Reactivity
Human, Rat, Mouse
Immunogen
Synthesized peptide derived from human PKR around the phosphorylation site of T451
Clonality
Polyclonal
Tag
Interferon-induced double-stranded RNA-activated protein kinase
Applications
IHC, IF, ELISA
Purification Method
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Shipping Conditions
Gel pack with blue ice.
Storage Conditions
Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Notes
Phospho-PKR (T451) Polyclonal Antibody detects endogenous levels of PKR protein only when phosphorylated at T451.
Formulation
Liquid solution
Buffer pH
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Applications Notes
Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC 1:100-1:300; ELISA 1:20000; IF 1:50-200
Other Product Names
EIF2AK2; PKR; PRKR; Interferon-induced; double-stranded RNA-activated protein kinase; Eukaryotic translation initiation factor 2-alpha kinase 2; eIF-2A protein kinase 2; Interferon-inducible RNA-dependent protein kinase; P1/eIF-2A protein k
Tested Applications
WB, IHC, IF, IP, ELISA
Host or Source
Rabbit
Accession Number URL
http://www.uniprot.org/uniprot/P19525
Isotype
Rabbit IgG
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