PP1α Polyclonal Antibody

Rabbit Anti-PP1α Polyclonal Antibody

Product Specifications

Background

The protein encoded by PPP1CA (protein phosphatase 1 catalytic subunit alpha) is one of the three catalytic subunits of protein phosphatase 1 (PP1) . PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Increased PP1 activity has been observed in the end stage of heart failure. Studies in both human and mice suggest that PP1 is an important regulator of cardiac function. Mouse studies also suggest that PP1 functions as a suppressor of learning and memory. Three alternatively spliced transcript variants encoding different isoforms have been found for PPP1CA.

CAS Number

9007-83-4

Product Name Alternative

PPP1CA; PPP1A; Serine/threonine-protein phosphatase PP1-alpha catalytic subunit; PP-1A

Gene ID

5499

Accession Number

P62136

Reactivity

Human, Mouse, Rat

Immunogen

Synthesized peptide derived from human PP1α around the non-phosphorylation site of T320

Clonality

Polyclonal

Tag

Serine threonine-protein phosphatase PP1-alpha catalytic subunit

Applications

WB, IHC, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

37kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

PP1α Polyclonal Antibody detects endogenous levels of PP1α protein.

Observed Molecular Weight

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:5000; IF 1:50-200