Arkadia Polyclonal Antibody

Rabbit Anti-Arkadia Polyclonal Antibody

Product Specifications

Background

The protein encoded by RNF111 (ring finger protein 111) is a nuclear RING-domain containing E3 ubiquitin ligase. This protein interacts with the transforming growth factor (TGF) -beta/NODAL signaling pathway by promoting the ubiquitination and proteosomal degradation of negative regulators, like SMAD proteins, and thereby enhances TGF-beta target-gene transcription. As a modulator of the nodal signaling cascade, RNF111 plays a critical role in the induction of mesoderm during embryonic development. Alternative splicing of RNF111 results in multiple transcript variants encoding distinct isoforms.

CAS Number

9007-83-4

Product Name Alternative

RNF111; E3 ubiquitin-protein ligase Arkadia; RING finger protein 111

Gene ID

54778

Accession Number

Q6ZNA4

Reactivity

Human, Mouse

Immunogen

Synthesized peptide derived from the C-terminal region of human Arkadia at AA range: 870-950

Clonality

Polyclonal

Tag

E3 ubiquitin-protein ligase Arkadia

Applications

WB, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

110kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Arkadia Polyclonal Antibody detects endogenous levels of Arkadia protein.

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; ELISA 1:40000; Not yet tested in other applications.