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ATP5F1 Polyclonal Antibody

Rabbit Anti-ATP5F1 Polyclonal Antibody

Product Specifications

Background

ATP5F1 (ATP synthase, H+ transporting, mitochondrial Fo complex subunit B1) encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8) . ATP5F1 encodes the b subunit of the proton channel.

CAS Number

9007-83-4

Product Name Alternative

ATP5F1; ATP synthase subunit b; mitochondrial; ATPase subunit b

Gene ID

515

Accession Number

P24539

Reactivity

Human, Mouse, Rat

Immunogen

Synthesized peptide derived from the Internal region of human ATP5F1 at AA range: 130-210

Clonality

Polyclonal

Tag

ATP synthase subunit b mitochondrial

Applications

WB, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

28kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

ATP5F1 Polyclonal Antibody detects endogenous levels of ATP5F1 protein.

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; ELISA 1:10000; Not yet tested in other applications.

Other Product Names

ATP5F1; ATP synthase subunit b; mitochondrial; ATPase subunit b

Tested Applications

WB, IHC, IF, IP, ELISA

Host or Source

Rabbit

Accession Number URL

http://www.uniprot.org/uniprot/P24539

Isotype

Rabbit IgG

Available Sizes

Curated Selection

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