Nephrin Polyclonal Antibody
Rabbit Anti-Nephrin Polyclonal Antibody
Product Specifications
Background
NPHS1 encodes a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. NPHS1 is primarily expressed in renal tissues, and the protein is a type-1 transmembrane protein found at the slit diaphragm of glomerular podocytes. The slit diaphragm is thought to function as an ultrafilter to exclude albumin and other plasma macromolecules in the formation of urine. Mutations in this gene result in Finnish-type congenital nephrosis 1, characterized by severe proteinuria and loss of the slit diaphragm and foot processes.
CAS Number
9007-83-4
Product Name Alternative
NPHS1; NPHN; Nephrin; Renal glomerulus-specific cell adhesion receptor
Gene ID
4868
Accession Number
O60500
Reactivity
Human, Mouse, Rat
Immunogen
Synthesized peptide derived from the C-terminal region of human Nephrin
Clonality
Polyclonal
Tag
Nephrin
Applications
WB, IHC, IF, ELISA
Purification Method
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Shipping Conditions
Gel pack with blue ice.
Storage Conditions
Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Notes
Nephrin Polyclonal Antibody detects endogenous levels of Nephrin protein.
Formulation
Liquid solution
Buffer pH
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Applications Notes
Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000; IHC 1:100-1:300; ELISA 1:40000; IF 1:50-200
Other Product Names
NPHS1; NPHN; Nephrin; Renal glomerulus-specific cell adhesion receptor
Tested Applications
WB, IHC, IF, IP, ELISA
Host or Source
Rabbit
Accession Number URL
http://www.uniprot.org/uniprot/O60500
Isotype
Rabbit IgG
Available Sizes
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