NFATc3 (phospho Ser165) Polyclonal Antibody

Rabbit Anti-NFATc3 (phospho Ser165) Polyclonal Antibody

Product Specifications

Background

The product of NFATC3 is a member of the nuclear factors of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation and an inducible nuclear component. Other members of this family participate to form this complex also. The product of NFATC3 plays a role in the regulation of gene expression in T cells and immature thymocytes. Several transcript variants encoding distinct isoforms have been identified for this gene.

CAS Number

9007-83-4

Product Name Alternative

NFATC3; NFAT4; Nuclear factor of activated T-cells; cytoplasmic 3; NF-ATc3; NFATc3; NFATx; T-cell transcription factor NFAT4; NF-AT4

Gene ID

4775

Accession Number

Q12968

Reactivity

Human, Mouse

Immunogen

Synthesized peptide derived from human NFATc3 around the phosphorylation site of S165

Clonality

Polyclonal

Tag

Nuclear factor of activated T-cells cytoplasmic 3

Applications

WB, IHC, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

115kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Phospho-NFATc3 (S165) Polyclonal Antibody detects endogenous levels of NFATc3 protein only when phosphorylated at S165.

Observed Molecular Weight

115

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:5000; Not yet tested in other applications.