ATP-citrate synthase (phospho Ser455) Polyclonal Antibody

Rabbit Anti-ATP-citrate synthase (phospho Ser455) Polyclonal Antibody

Product Specifications

Background

ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Multiple transcript variants encoding distinct isoforms have been identified for this gene.

CAS Number

9007-83-4

Product Name Alternative

ACLY; ATP-citrate synthase; ATP-citrate; pro-S-) -lyase; ACL; Citrate cleavage enzyme

Gene ID

Accession Number

P53396

Reactivity

Human, Mouse, Rat, Monkey

Immunogen

Synthesized peptide derived from human ATP-citrate synthase around the phosphorylation site of S455

Clonality

Polyclonal

Tag

ATP-citrate synthase

Applications

WB, IHC, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

125kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Phospho-ATP-citrate synthase (S455) Polyclonal Antibody detects endogenous levels of ATP-citrate synthase protein only when phosphorylated at S455.

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:10000; IF 1:50-200