Nibrin (phospho Ser278) Polyclonal Antibody

Rabbit Anti-Nibrin (phospho Ser278) Polyclonal Antibody

Product Specifications

Background

Mutations in NBN are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. This gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation.

CAS Number

9007-83-4

Product Name Alternative

NBN; NBS; NBS1; P95; Nibrin; Cell cycle regulatory protein p95; Nijmegen breakage syndrome protein 1

Gene ID

4683

Accession Number

O60934

Reactivity

Human, Rat, Mouse

Immunogen

Synthesized peptide derived from human Nibrin around the phosphorylation site of S278

Clonality

Polyclonal

Tag

Nibrin

Applications

WB, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

95kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Phospho-Nibrin (S278) Polyclonal Antibody detects endogenous levels of Nibrin protein only when phosphorylated at S278.

Observed Molecular Weight

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IF 1:200-1:1000; ELISA 1:10000; Not yet tested in other applications.