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NACA (phospho Ser43) Polyclonal Antibody

Rabbit Anti-NACA (phospho Ser43) Polyclonal Antibody

Product Specifications

Background

NACA encodes a protein that associates with basic transcription factor 3 (BTF3) to form the nascent polypeptide-associated complex (NAC) . This complex binds to nascent proteins that lack a signal peptide motif as they emerge from the ribosome, blocking interaction with the signal recognition particle (SRP) and preventing mistranslocation to the endoplasmic reticulum. This protein is an IgE autoantigen in atopic dermatitis patients. Alternative splicing results in multiple transcript variants, but the full length nature of some of these variants, including those encoding very large proteins, has not been determined. There are multiple pseudogenes of NACA on different chromosomes.

CAS Number

9007-83-4

Product Name Alternative

NACA; HSD48; Nascent polypeptide-associated complex subunit alpha; NAC-alpha; Alpha-NAC; allergen Hom s 2

Gene ID

4666

Accession Number

Q13765

Reactivity

Human, Mouse, Rat

Immunogen

Synthesized peptide derived from human NACA around the phosphorylation site of S43

Clonality

Polyclonal

Tag

Nascent polypeptide-associated complex subunit alpha

Applications

IHC, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Phospho-NACA (S43) Polyclonal Antibody detects endogenous levels of NACA protein only when phosphorylated at S43.

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC 1:100-1:300; ELISA 1:5000; IF 1:50-200

Other Product Names

NACA; HSD48; Nascent polypeptide-associated complex subunit alpha; NAC-alpha; Alpha-NAC; allergen Hom s 2

Tested Applications

WB, IHC, IF, IP, ELISA

Host or Source

Rabbit

Accession Number URL

http://www.uniprot.org/uniprot/Q13765

Isotype

Rabbit IgG

Available Sizes

Frequently Asked Questions

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