C-Myc (phospho Ser373) Polyclonal Antibody

Rabbit Anti-c-Myc (phospho Ser373) Polyclonal Antibody

Product Specifications

Background

The protein encoded by MYC is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene.

CAS Number

9007-83-4

Product Name Alternative

MYC; BHLHE39; Myc proto-oncogene protein; Class E basic helix-loop-helix protein 39; bHLHe39; Proto-oncogene c-Myc; Transcription factor p64

Gene ID

4609

Accession Number

P01106

Reactivity

Human, Mouse, Rat

Immunogen

Synthesized peptide derived from human c-Myc around the phosphorylation site of S373

Clonality

Polyclonal

Tag

Myc proto-oncogene protein

Applications

IHC, IF, IP, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

50kD, (also ~60kD in some samples)

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Phospho-c-Myc (S373) Polyclonal Antibody detects endogenous levels of c-Myc protein only when phosphorylated at S373.

Observed Molecular Weight

50, (also ~60KD in some samples)

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC 1:100-1:300; IP 2-5 ug:mg lysate; ELISA 1:20000; IF 1:50-200