C-Myb (phospho Ser12) Polyclonal Antibody

Rabbit Anti-c-Myb (phospho Ser12) Polyclonal Antibody

Product Specifications

Background

MYB encodes a protein with three HTH DNA-binding domains that functions as a transcription regulator. This protein plays an essential role in the regulation of hematopoiesis. MYB may be aberrently expressed or rearranged or undergo translocation in leukemias and lymphomas, and is considered to be an oncogene. Alternative splicing results in multiple transcript variants.

CAS Number

9007-83-4

Product Name Alternative

MYB; Transcriptional activator Myb; Proto-oncogene c-Myb

Gene ID

4602

Accession Number

P10242

Reactivity

Human, Mouse

Immunogen

Synthesized peptide derived from human c-Myb around the phosphorylation site of S12

Clonality

Polyclonal

Tag

Transcriptional activator Myb

Applications

WB, IHC, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

65kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Phospho-c-Myb (S12) Polyclonal Antibody detects endogenous levels of c-Myb protein only when phosphorylated at S12.

Observed Molecular Weight

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:5000; IF 1:50-200