Anti-Hu CD40 Purified

CD40 is a costimulatory molecule of the TNF receptor superfamily and is expressed on many cell types, such as B cells, monocytes/macrophages, dendritic cells, endothelial cells, fibroblasts or vascular smooth muscle cells. Interaction of CD40 and its ligand CD154 (CD40L) is required for the generation of antibody responses to T-dependent antigens as well as for the development of germinal centers and memory B cells. In monocytes/macrophages CD40 engagement induces production of pro-inflammatory cytokines and chemokines. CD40-CD154 interactions are also critical for development of CD4 T cell-dependent effector functions. CD40 links innate and adaptive immune responses to bacterial stimuli and serves as an important regulator affecting functions of other costimulatory molecules.

Product Specifications

Certification

RUO

Reactivity

Non-human primates, Human

Immunogen

Human B lymphoblastoid cell lines

Target Antigen

CD40

Clone

HB14

Applications

ICC, FC (QC tested), IHC

Concentration

1 mg/mL

Format

Purified

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995) ., URL: , *Kansas GS, Tedder TF: Transmembrane signals generated through MHC class II, CD19, CD20, CD39, and CD40 antigens induce LFA-1-dependent and independent adhesion in human B cells through a tyrosine kinase-dependent pathway. J Immunol. 1991 Dec 15;147 (12) :4094-102., URL: https://www.ncbi.nlm.nih.gov/pubmed/1721639

Other References

*Oxenius A, Campbell KA, Maliszewski CR, Kishimoto T, Kikutani H, Hengartner H, Zinkernagel RM, Bachmann MF. CD40-CD40 ligand interactions are critical in T-B cooperation but not for other anti-viral CD4+ T cell functions. J Exp Med. 1996 May 1;183 (5) :2209-18., URL: https://www.ncbi.nlm.nih.gov/pubmed/8642330?ordina, *Grewal IS, Flavell RA: The role of CD40 ligand in costimulation and T-cell activation. Immunol Rev. 1996 Oct;153:85-106., URL: https://www.ncbi.nlm.nih.gov/pubmed/9010720?ordina, *Pearson LL, Castle BE, Kehry MR: CD40-mediated signaling in monocytic cells: up-regulation of tumor necrosis factor receptor-associated factor mRNAs and activation of mitogen-activated protein kinase signaling pathways. Int Immunol. 2001 Mar;13 (3) :273-83., URL: https://www.ncbi.nlm.nih.gov/pubmed/11222496?ordin, *Wu W, Alexis NE, Chen X, Bromberg PA, Peden DB: Involvement of mitogen-activated protein kinases and NFkappaB in LPS-induced CD40 expression on human monocytic cells. Toxicol Appl Pharmacol. 2007 Dec 14, URL: https://www.ncbi.nlm.nih.gov/pubmed/18187173?ordin

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The mouse monoclonal antibody HB14 recognizes an extracellular epitope of CD40 (BP50), a 48 kDa type I single chain transmembrane glycoprotein expressed on normal and neoplastic B cells, but not on terminally differentiated plasma cells. CD40 antigen is also present on Hodgkin's and Reed-Sternberg cells, follicular dendritic cells, some macrophages, basal epithelial cells and endothelial cells.