HSL (phospho Ser552) Polyclonal Antibody

Rabbit Anti-HSL (phospho Ser552) Polyclonal Antibody

Product Specifications

Background

The protein encoded by LIPE has a long and a short form, generated by use of alternative translational start codons. The long form is expressed in steroidogenic tissues such as testis, where it converts cholesteryl esters to free cholesterol for steroid hormone production. The short form is expressed in adipose tissue, among others, where it hydrolyzes stored triglycerides to free fatty acids.

CAS Number

9007-83-4

Product Name Alternative

LIPE; Hormone-sensitive lipase; HSL

Gene ID

3991

Accession Number

Q05469

Reactivity

Human, Mouse, Rat

Immunogen

Synthesized peptide derived from human HSL around the phosphorylation site of S552

Clonality

Polyclonal

Tag

Hormone-sensitive lipase

Applications

WB, IHC, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

117kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Phospho-HSL (S552) Polyclonal Antibody detects endogenous levels of HSL protein only when phosphorylated at S552.

Observed Molecular Weight

117

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:5000; IF 1:50-200