NCAM-L1 (phospho Ser1181) Polyclonal Antibody

Rabbit Anti-NCAM-L1 (phospho Ser1181) Polyclonal Antibody

Product Specifications

Background

The protein encoded by L1CAM is an axonal glycoprotein belonging to the immunoglobulin supergene family. The ectodomain, consisting of several immunoglobulin-like domains and fibronectin-like repeats (type III), is linked via a single transmembrane sequence to a conserved cytoplasmic domain. This cell adhesion molecule plays an important role in nervous system development, including neuronal migration and differentiation. Mutations in the gene cause X-linked neurological syndromes known as CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus) . Alternative splicing of L1CAM results in multiple transcript variants, some of which include an alternate exon that is considered to be specific to neurons.

CAS Number

9007-83-4

Product Name Alternative

L1CAM; CAML1; MIC5; Neural cell adhesion molecule L1; N-CAM-L1; NCAM-L1; CD antigen CD171

Gene ID

3897

Accession Number

P32004

Reactivity

Human, Mouse, Rat

Immunogen

Synthesized peptide derived from human NCAM-L1 around the phosphorylation site of S1181

Clonality

Polyclonal

Tag

Neural cell adhesion molecule L1

Applications

WB, IHC, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

180kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Phospho-NCAM-L1 (S1181) Polyclonal Antibody detects endogenous levels of NCAM-L1 protein only when phosphorylated at S1181.

Observed Molecular Weight

180

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:20000; IF 1:50-200