Cytokeratin 14/16 Polyclonal Antibody

Rabbit Anti-Cytokeratin 14/16 Polyclonal Antibody

Product Specifications

Background

KRT14 encodes a member of the keratin family, the most diverse group of intermediate filaments. This gene product, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. At least one pseudogene has been identified at 17p12-p11.

CAS Number

9007-83-4

Product Name Alternative

KRT14; Keratin; type I cytoskeletal 14; Cytokeratin-14; CK-14; Keratin-14; K14; KRT16; KRT16A; Keratin, type I cytoskeletal 16; Cytokeratin-16; CK-16; Keratin-16; K16

Gene ID

3861/3868/3872

Accession Number

P02533/P08779

Reactivity

Human, Mouse, Rat

Immunogen

Synthesized peptide derived from the N-terminal region of human Cytokeratin 14/16 at AA rangle: 1-80

Clonality

Polyclonal

Tag

Keratin type I cytoskeletal 14

Applications

WB, IHC, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

52kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Cytokeratin 14/16 Polyclonal Antibody detects endogenous levels of Cytokeratin 14/16 protein.

Observed Molecular Weight

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:20000; Not yet tested in other applications.

Other Product Names

KRT14; Keratin; type I cytoskeletal 14; Cytokeratin-14; CK-14; Keratin-14; K14; KRT16; KRT16A; Keratin, type I cytoskeletal 16; Cytokeratin-16; CK-16; Keratin-16; K16