Flk-1/Flt-4 Polyclonal Antibody

Rabbit Anti-Flk-1/Flt-4 Polyclonal Antibody

Product Specifications

Background

VEGF R1 is one of the five receptor tyrosine kinases (RTKs) (Flt-1, KDR/Flk-1, Flt-4, tie-1, and tek/tie-2) whose expression is almost exclusively restricted to the endothelial cells. Tie-1 and tek/tie-2 define a new class of RTKs containing two immunoglobulin-like domains, three EGF homology domains and three fibronectin type III domains in their extracellular regions. Flt-1, KDR/Flk-1 and Flt-4 are members of the class III subfamily of RTKs containing seven immunoglobulin-like repeats in their extracellular domains. All five RTKs are likely to play central roles in vasculogenesis and angiogenesis.

CAS Number

9007-83-4

Product Name Alternative

KDR; FLK1; VEGFR2; Vascular endothelial growth factor receptor 2; VEGFR-2; Fetal liver kinase 1; FLK-1; Kinase insert domain receptor; KDR; Protein-tyrosine kinase receptor flk-1; CD antigen CD309; FLT4; VEGFR3; Vascular endothelial growth

Gene ID

3791/2324

Accession Number

P35968/P35916

Reactivity

Human, Mouse, Rat

Immunogen

Synthesized peptide derived from human Flk-1/Flt-4 around the non-phosphorylation site of Y1054/Y1063

Clonality

Polyclonal

Tag

Vascular endothelial growth factor receptor 2

Applications

IHC, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Flk-1/Flt-4 Polyclonal Antibody detects endogenous levels of Flk-1/Flt-4 protein.

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:40000; Not yet tested in other applications.