AP-1 (phospho Ser243) Polyclonal Antibody

Rabbit Anti-AP-1 (phospho Ser243) Polyclonal Antibody

Product Specifications

Background

JUN is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies.

CAS Number

9007-83-4

Product Name Alternative

JUN; Transcription factor AP-1; Activator protein 1; AP1; Proto-oncogene c-Jun; V-jun avian sarcoma virus 17 oncogene homolog; p39

Gene ID

3725

Accession Number

P05412

Reactivity

Human, Mouse, Rat

Immunogen

Synthesized peptide derived from human AP-1 around the phosphorylation site of S243

Clonality

Polyclonal

Tag

Transcription factor AP-1

Applications

WB, IHC, IF, IP, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

39-42kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Phospho-AP-1 (S243) Polyclonal Antibody detects endogenous levels of AP-1 protein only when phosphorylated at S243.

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; IP 2-5 ug:mg lysate; ELISA 1:20000; IF 1:50-200