IRAK-1 (phospho Thr100) Polyclonal Antibody

Rabbit Anti-IRAK-1 (phospho Thr100) Polyclonal Antibody

Product Specifications

Background

IRAK1 encodes the interleukin-1 receptor-associated kinase 1, one of two putative serine/threonine kinases that become associated with the interleukin-1 receptor (IL1R) upon stimulation. This gene is partially responsible for IL1-induced upregulation of the transcription factor NF-kappa B. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

CAS Number

9007-83-4

Product Name Alternative

IRAK1; IRAK; Interleukin-1 receptor-associated kinase 1; IRAK-1

Gene ID

3654

Accession Number

P51617

Reactivity

Human, Mouse, Rat

Immunogen

Synthesized peptide derived from human IRAK-1 around the phosphorylation site of T100

Clonality

Polyclonal

Tag

Interleukin-1 receptor-associated kinase 1

Applications

WB, IHC, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

77kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Phospho-IRAK-1 (T100) Polyclonal Antibody detects endogenous levels of IRAK-1 protein only when phosphorylated at T100.

Observed Molecular Weight

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:20000; IF 1:50-200